Integrated gene-free potato genome editing using transient transcription activator-like effector nucleases and regeneration-promoting gene expression by <i>Agrobacterium</i> infection

Genome editing is highly useful for crop improvement. The method of expressing genome-editing enzymes using a transient expression system in Agrobacterium, called agrobacterial mutagenesis, shortcut used technology to improve elite varieties vegetatively propagated crops, including potato. However, with this method, edited individuals cannot be selected. regeneration-promoting genes can result shoot regeneration from plantlets, while the constitutive most does not normally regenerated shoots. Here, we report that could obtain genome-edited potatoes by positive selection. These shoots were obtained via combined gene enzyme gene. Moreover, confirmed did contain sequence binary vector Agrobacterium. Our data have been submitted Japanese regulatory authority, Ministry Education, Culture, Sports, Science and Technology (MEXT), are process conducting field tests further research on these potatoes. work presents powerful regarding acquisition crops through